THREE-DIMENSIONAL CULTURE SCAFFOLDS FOR OSTEOSARCOMA CANCER STEM CELLS

Received 2023-07-13; Accepted 2023-09-26; Published 2023-09-26

Authors

  • Halimatun Saadiah Abdul Wahab Department of Basic Sciences, Faculty of Health Sciences, UiTM Cawangan Selangor Campus Puncak Alam, Bandar Puncak Alam, 42300 Puncak Alam, Selangor.
  • Aisha Mohd Din Department of Basic Sciences, Faculty of Health Sciences, UiTM Cawangan Selangor Campus Puncak Alam, Bandar Puncak Alam, 42300 Puncak Alam, Selangor.
  • Gabriele Ruth Anisah Froemming Centre of Preclinical Sciences, Faculty of Dentistry, UiTM Cawangan Selangor, Campus Sungai Buloh, Jalan Hospital, 47000 Sungai Buloh, Selangor.
  • Hairil Rashmizal Abdul Razak Medical Imaging Programme, Department of Health and Care Professions, Faculty of Health and Life Sciences, University of Exeter, EX1 2LU Devon, United Kingdom.
  • Sharaniza Ab. Rahim Department of Biochemistry and Molecular Medicine, Faculty of Medicine, UiTM Cawangan Selangor, Campus Sungai Buloh, Jalan Hospital, 47000 Sungai Buloh, Selangor.
  • Amir Muhriz Abdul Latiff Department of Pathology, Faculty of Medicine, UiTM Cawangan Selangor, Campus Sungai Buloh, Jalan Hospital, 47000 Sungai Buloh, Selangor.

DOI:

https://doi.org/10.22452/jummec.sp2023no2.51

Keywords:

3D Culture, Osteosarcoma CSCs, Scaffold, Stemness

Abstract

Cancer stem cells (CSCs) are a subpopulation of tumour cells that are highly tumorigenic with self-renewing potential. In osteosarcoma, these cells are responsible for drug resistance and cancer relapse. Studying CSCs in vitro can provide a better development of therapeutic strategies by understanding the mechanism of tumorigenesis and chemoresistance in osteosarcoma. Cell culture plays a crucial role in cancer research, stem cell studies, and drug discovery. While two-dimensional (2D) methods are commonly used for cell culturing, recent advancements in three-dimensional (3D) techniques offer promising opportunities for conducting complex experiments. With 3D cell culture, the cellular environment can be manipulated to closely mimic in vivo conditions, resulting in more
accurate data about cell-to-cell interactions and tumour characteristics. Various scaffold-based techniques using (1) natural polymers such as hydrogel, collagen type I, agar gel, Matrigel, alginate, bacterial cellulose, hyaluronic acid, and (2) synthetic polymers such as polyethylene glycol diacrylate (PEGDA) and poly (2-hydroxyethyl methacrylate) (pHEMA) offer unique advantages and applications for studying osteosarcoma CSCs. Scaffold-free techniques such as ultra-low binding plates and hanging drop are also used to culture osteosarcoma CSCs. This review article describes
various 3D culture methods used in forming osteosarcoma CSC spheroids and the expression of stemness markers.

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Author Biographies

Halimatun Saadiah Abdul Wahab, Department of Basic Sciences, Faculty of Health Sciences, UiTM Cawangan Selangor Campus Puncak Alam, Bandar Puncak Alam, 42300 Puncak Alam, Selangor.

 

 

 

Gabriele Ruth Anisah Froemming, Centre of Preclinical Sciences, Faculty of Dentistry, UiTM Cawangan Selangor, Campus Sungai Buloh, Jalan Hospital, 47000 Sungai Buloh, Selangor.

 

 

 

Hairil Rashmizal Abdul Razak, Medical Imaging Programme, Department of Health and Care Professions, Faculty of Health and Life Sciences, University of Exeter, EX1 2LU Devon, United Kingdom.

 

 

Sharaniza Ab. Rahim, Department of Biochemistry and Molecular Medicine, Faculty of Medicine, UiTM Cawangan Selangor, Campus Sungai Buloh, Jalan Hospital, 47000 Sungai Buloh, Selangor.

 

 

Amir Muhriz Abdul Latiff, Department of Pathology, Faculty of Medicine, UiTM Cawangan Selangor, Campus Sungai Buloh, Jalan Hospital, 47000 Sungai Buloh, Selangor.

 

 

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Published

2023-09-26